自己的单细胞数据加测了TCR/BCR，想和公共数据集的单细胞数据合并分析，但是，公共数据集的单细胞数据没有加测TCR/BCR，这样肯定是不能直接合并分析。
这篇文章，提供了一个可行的思路
文献解决方案
使用正则表达式分别去除BCR/TCR基因
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BCR-genes were removed from the count data using regular expression commands with the following patterns: ‘IG[HKL]V’, ‘IG[KL]J’, ‘IG[KL]C’, ‘IGH[ADEGM]’.
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TCR-genes were removed from the count data using a regular expression command with the following pattern:’^TR[ABDG][VJC]’. A cluster containing cells positive for CD3E, CD19 and CD14 was discarded.
文献主要结论
文章中作者解决的问题是BCR/TCR会对聚类结果有影响，而不是我最开始提出的问题。😂误打误撞了
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the genes that encode B-cell antigen receptors interfere with the process of unsupervised clustering, as well as the down-stream analyses of these cells.
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the genes that encode T-cell receptors interfere with the unsupervised clustering of these cells.
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This interference is likely due to the high frequencies of B- and T-cell receptor genes among the genes that account for most of the variance in each of the PCs used for the clustering.
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The effects of the B-cell and T-cell receptor genes are abrogated upon their exclusion before clustering is undertaken.
并且还提供了代码
Code availability
All custom codes used for data processing and computational analyses are available under project BCR_TCR_Interference at https://github.com/MartenssonLab/.